Isolation of Shiga toxin-producing Escherichia coli harboring variant Shiga toxin genes from seafood.

ABSTRACT:

Background and aim

Shiga toxin-producing Escherichia coli (STEC) are important pathogens of global significance. STEC are responsible for numerous food-borne outbreaks worldwide and their presence in food is a potential health hazard. The objective of the present study was to determine the incidence of STEC in fresh seafood in Mumbai, India, and to characterize STEC with respect to their virulence determinants.

Materials and methods

A total of 368 E. coli were isolated from 39 fresh seafood samples (18 finfish and 21 shellfish) using culture-based methods. The isolates were screened by polymerase chain reaction (PCR) for the genes commonly associated with STEC. The variant Shiga toxin genes were confirmed by Southern blotting and hybridization followed by DNA s

SUBMITTER: Prakasan S 

PROVIDER: S-EPMC5891856 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature