Stromal TRIM28-associated signaling pathway modulation within the colorectal cancer microenvironment.

ABSTRACT:

Background

Stromal gene expression patterns predict patient outcomes in colorectal cancer. TRIM28 is a transcriptional co-repressor that regulates an abundance of genes through the KRAB domain family of transcription factors. We have previously shown that stromal expression of TRIM28 is a marker of disease relapse and poor survival in colorectal cancer. Here, we perform differential epithelium-stroma proteomic network analyses to characterize signaling pathways associated with TRIM28 within the tumor microenvironment.

Methods

Reverse phase protein arrays were generated from laser capture micro-dissected carcinoma and stromal cells from fresh frozen colorectal cancer tissues. Phosphorylation and total protein levels were measured for 30 cancer-related signaling pathway endpoi

SUBMITTER: Fitzgerald S 

PROVIDER: S-EPMC5891886 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature