Safety and tracking of intrathecal allogeneic mesenchymal stem cell transplantation in healthy and diseased horses.

ABSTRACT:

Background

It is currently unknown if the intrathecal administration of a high dose of allogeneic mesenchymal stem cells (MSCs) is safe, how MSCs migrate throughout the vertebral canal after intrathecal administration, and whether MSCs are able to home to a site of injury. The aims of the study were: 1) to evaluate the safety of intrathecal injection of 100 million allogeneic adipose-derived MSCs (ASCs); 2) to assess the distribution of ASCs after atlanto-occipital (AO) and lumbosacral (LS) injection in healthy horses; and 3) to determine if ASCs homed to the site of injury in neurologically diseased horses.

Methods

Six healthy horses received 100 × 10⁶ allogeneic ASCs via AO (n = 3) or LS injection (n = 3). For two of these horses, ASCs were radiolabeled with tec

SUBMITTER: Barberini DJ 

PROVIDER: S-EPMC5891950 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature