Structure and function of a novel periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria.

ABSTRACT: Periplasmic solute-binding proteins in bacteria are involved in the active transport of nutrients into the cytoplasm. In marine bacteria of the genus Vibrio, a chitooligosaccharide-binding protein (CBP) is thought to be the major solute-binding protein controlling the rate of chitin uptake in these bacteria. However, the molecular mechanism of the CBP involvement in chitin metabolism has not been elucidated. Here, we report the structure and function of a recombinant chitooligosaccharide-binding protein from Vibrio harveyi, namely VhCBP, expressed in Escherichia coli Isothermal titration calorimetry revealed that VhCBP strongly binds shorter chitooligosaccharides ((GlcNAc) _n , where n = 2, 3, and 4) with affinities that are consid

SUBMITTER: Suginta W 

PROVIDER: S-EPMC5892562 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature