Metformin inhibits proliferation and cytotoxicity and induces apoptosis via AMPK pathway in CD19-chimeric antigen receptor-modified T cells.

ABSTRACT:

Background

CD19-chimericantigen receptor (CAR) modified T cells (CD19-CAR T cells) have been well documented to possess potent anti-tumor properties against CD19-expressingleukemia cells. As a traditional medicine, metformin has been widely used to treat type II diabetes mellitus and more recently has become a candidate for the treatment of cancer. However, no report has revealed the direct effect of metformin on CD19-CAR T cell biological function and its underling mechanisms.

Purpose

The purpose of this research was to explore the effect of metformin on CD19-CAR T cell biological function and the mechanisms involved.

Methods

CD19-CAR T cells proliferation, apoptosis and cytotoxicity were mainly tested by CCK-8 assay, flow cytometry and ELISA. The detection of mechan

SUBMITTER: Mu Q 

PROVIDER: S-EPMC5892609 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature