A conserved threonine prevents self-intoxication of enoyl-thioester reductases.

ABSTRACT: Enzymes are highly specific biocatalysts, yet they can promote unwanted side reactions. Here we investigated the factors that direct catalysis in the enoyl-thioester reductase Etr1p. We show that a single conserved threonine is essential to suppress the formation of a side product that would otherwise act as a high-affinity inhibitor of the enzyme. Substitution of this threonine with isosteric valine increases side-product formation by more than six orders of magnitude, while decreasing turnover frequency by only one order of magnitude. Our results show that the promotion of wanted reactions and the suppression of unwanted side reactions operate independently at the active site of Etr1p, and that the active suppression of side reactions is highly conserved in the family of medium-chain deh

SUBMITTER: Rosenthal RG 

PROVIDER: S-EPMC5892710 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature