Identification of high affinity HER2 binding antibodies using CHO Fab surface display.

ABSTRACT: Discovery of monoclonal antibodies is most commonly performed using phage or yeast display but mammalian cells are used for production because of the complex antibody structure, including the multiple disulfide bonds and glycosylation, required for function. As this transition between host organisms is often accompanied by impaired binding, folding or expression, development pipelines include laborious plate-based screening or engineering strategies to adapt an antibody to mammalian expression. To circumvent these problems, we developed a plasmid-based Fab screening platform on Chinese hamster ovary (CHO) cells which allows for antibody selection in the production host and in the presence of the same post-translational modifications as the manufactured product. A hu4D5 variant with low aff

SUBMITTER: Nguyen AW 

PROVIDER: S-EPMC5892863 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature