Photochemical degradation of trypan blue.

ABSTRACT:

Purpose

To investigate the photochemical degradation of trypan blue (TB) and to identify decomposition products.

Methods

Defined solution samples of TB and a mixture with lutein/zeaxanthin were exposed to blue light. Thermal degradation processes were ruled out using controls not subjected to irradiation. All samples were analyzed using optical microscopy, UV/Vis spectroscopy, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and nuclear magnetic resonance (NMR) spectrometry. Degradation kinetics were determined based on changes in absorbance; intermediates were identified by analyzing mass differences of characteristic fragment ion peaks within the fragmentation patterns, and assignments were verified by NMR.

Results

TB demonstr

SUBMITTER: Brockmann T 

PROVIDER: S-EPMC5892916 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature