Distal airway microbiome is associated with immunoregulatory myeloid cell responses in lung transplant recipients.

ABSTRACT: Long-term survival of lung transplant recipients (LTRs) is limited by the occurrence of bronchiolitis obliterans syndrome (BOS). Recent evidence suggests a role for microbiome alterations in the occurrence of BOS, although the precise mechanisms are unclear. In this study we evaluated the relationship between the airway microbiome and distinct subsets of immunoregulatory myeloid-derived suppressor cells (MDSCs) in LTRs. Bronchoalveolar lavage (BAL) and simultaneous oral wash and nasal swab samples were collected from adult LTRs. Microbial genomic DNA was isolated, 16S rRNA genes amplified using V4 primers, and polymerase chain reaction (PCR) products sequenced and analyzed. BAL MDSC subsets were enumerated using flow cytometry. The oral microbiome signature differs from that of the nasal,

SUBMITTER: Sharma NS 

PROVIDER: S-EPMC5893420 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature