Towards an in vitro fibrogenesis model of human vocal fold scarring.

ABSTRACT:

Background

Vocal fold (VF) scarring remains a therapeutic dilemma and challenge in modern laryngology. To facilitate corresponding research, we aimed to establish an in vitro fibrogenesis model employing human VF fibroblasts (hVFF) and the principles of macromolecular crowding (MMC).

Methods

Fibrogenesis was promoted by addition of transforming growth factor-β1 to standard medium and medium containing inert macromolecules (MMC). Hepatocyte growth factor (HGF) and Botox type A were tested for their antifibrotic properties in various doses. Experiments were analyzed with respect to the biosynthesis of collagen, fibronectin, and α-smooth muscle actin using immunofluorescence, silver stain and western blot.

Results

MMC led to favourable enhanced deposition of collagen and

SUBMITTER: Graupp M 

PROVIDER: S-EPMC5893733 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature