Inferring the Minimal Genome of <i>Mesoplasma florum</i> by Comparative Genomics and Transposon Mutagenesis.

ABSTRACT: The creation and comparison of minimal genomes will help better define the most fundamental mechanisms supporting life. Mesoplasma florum is a near-minimal, fast-growing, nonpathogenic bacterium potentially amenable to genome reduction efforts. In a comparative genomic study of 13 M. florum strains, including 11 newly sequenced genomes, we have identified the core genome and open pangenome of this species. Our results show that all of the strains have approximately 80% of their gene content in common. Of the remaining 20%, 17% of the genes were found in multiple strains and 3% were unique to any given strain. On the basis of random transposon mutagenesis, we also estimated that ~290 out of 720 genes are essential for M. florum L1 in rich medium. We next evaluated different genome reduction

SUBMITTER: Baby V 

PROVIDER: S-EPMC5893858 | biostudies-literature | 2018 May-Jun

REPOSITORIES: biostudies-literature