Detection and genotyping of Trypanosoma cruzi from acai products commercialized in Rio de Janeiro and Para, Brazil.

ABSTRACT:

Background

Several cases of food-borne acute Chagas disease (ACD) have been reported in the Brazilian Amazon so far. Up to 2004, the occurrence of ACD by oral transmission, associated with food consumption, was rare. Recent cases of ACD in Brazil have been attributed to the consumption of juice from the açai palm containing reservoir animals or insect vectors waste, infected with Trypanosoma cruzi. This study aimed to determine the T. cruzi contamination rate and to genotype the parasite in food samples prepared from açai, which are commercialized in Rio de Janeiro and the Pará States in Brazil.

Methods

The amplificability of DNA extracted from açai samples, and T. cruzi and Triatominae detection were performed by conventional PCR. Molecular characterization was done by mult

SUBMITTER: Ferreira RTB 

PROVIDER: S-EPMC5894193 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature