Metformin restores electrophysiology of small conductance calcium-activated potassium channels in the atrium of GK diabetic rats.

ABSTRACT:

Background

Small conductance calcium-activated potassium channels (SK channels) play a critical role in action potential repolarization in cardiomyocytes. Recently, the potential anti-arrhythmic effect of metformin in diabetic patients has been recognized, yet the underlying mechanism remains elusive.

Methods

Diabetic Goto-Kakizaki (GK) rats were untreated or treated with metformin (300 mg/kg/day) for 12 weeks, and age-matched Wistar rats were used as control (n = 6 per group). Electrocardiography, Hematoxylin-eosin staining and Masson's trichome staining were performed to assess cardiac function, histology and fibrosis. The expression levels of the SK channels in the myocardium were determined by real-time PCR and Western blotting. The electrophysiology of the SK channels i

SUBMITTER: Fu X 

PROVIDER: S-EPMC5894224 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature