Identification of Infectious Bursal Disease Virus with Atypical VP2 Amino Acid Profile in Latvia.

ABSTRACT:

Introduction

Infectious bursal disease virus (IBDV) is a causative agent of immunosuppressive disorder resulting in significant losses to the world poultry industry. This study describes the molecular characterisation of an atypical IBDV from a field outbreak that occurred in vaccinated chicken flocks in Latvia in 2011.

Material and methods

Ten bursae of Fabricius from each flock were collected for laboratory examination. Virus isolation was performed in embryonated eggs and CEF culture. The RT-PCR aimed at hypervariable domain of VP2 gene combined with sequencing was performed for detection and identification of IBDV.

Results

The molecular examinations confirmed the IBDV infection. The analysis of the amino acid sequence revealed that the strain possessed four amino

SUBMITTER: Pikula A 

PROVIDER: S-EPMC5894395 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature