Tools to minimize interlaboratory variability in vitellogenin gene expression monitoring programs.

ABSTRACT: The egg yolk precursor protein vitellogenin is widely used as a biomarker of estrogen exposure in male fish. However, standardized methodology is lacking and little is known regarding the reproducibility of results among laboratories using different equipment, reagents, protocols, and data analysis programs. To address this data gap we tested the reproducibility across laboratories to evaluate vitellogenin gene (vtg) expression and assessed the value of using a freely available software data analysis program. Samples collected from studies of male fathead minnows (Pimephales promelas) exposed to 17α-ethinylestradiol (EE2) and minnows exposed to processed wastewater effluent were evaluated for vtg expression in 4 laboratories. Our results indicate reasonable consistency among laboratories i

SUBMITTER: Jastrow A 

PROVIDER: S-EPMC5894818 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature