Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture.

ABSTRACT: Glioblastoma multiforme (GBM) is an aggressive incurable brain cancer. The cells that fuel the growth of tumours resemble neural stem cells found in the developing and adult mammalian forebrain. These are referred to as glioma stem cells (GSCs). Similar to neural stem cells, GSCs exhibit a variety of phenotypic states: dormant, quiescent, proliferative and differentiating. How environmental cues within the brain influence these distinct states is not well understood. Laboratory models of GBM can be generated using either genetically engineered mouse models, or via intracranial transplantation of cultured tumour initiating cells (mouse or human). Unfortunately, these approaches are expensive, time-consuming, low-throughput and ill-suited for monitoring live cell behaviours. Here, we explore

SUBMITTER: Marques-Torrejon MA 

PROVIDER: S-EPMC5894940 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature