High-throughput screen for compounds that modulate neurite growth of human induced pluripotent stem cell-derived neurons.

ABSTRACT: Development of technology platforms to perform compound screens of human induced pluripotent stem cell (hiPSC)-derived neurons with relatively high throughput is essential to realize their potential for drug discovery. Here, we demonstrate the feasibility of high-throughput screening of hiPSC-derived neurons using a high-content, image-based approach focused on neurite growth, a process that is fundamental to formation of neural networks and nerve regeneration. From a collection of 4421 bioactive small molecules, we identified 108 hit compounds, including 37 approved drugs, that target molecules or pathways known to regulate neurite growth, as well as those not previously associated with this process. These data provide evidence that many pathways and targets known to play roles in neurite

SUBMITTER: Sherman SP 

PROVIDER: S-EPMC5894944 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature