Snf1/AMPK is involved in the mitotic spindle alignment in Saccharomyces cerevisiae.

ABSTRACT: Before anaphase onset, budding yeast cells must align the mitotic spindle parallel to the mother-bud axis to ensure proper chromosome segregation. The protein kinase Snf1/AMPK is a highly conserved energy sensor, essential for adaptation to glucose limitation and in response to cellular stresses. However, recent findings indicate that it plays important functions also in non-limiting glucose conditions. Here we report a novel role of Snf1/AMPK in the progression through mitosis in glucose-repressing condition. We show that active Snf1 is localized to the bud neck from bud emergence to cytokinesis in a septin-dependent manner. In addition, loss of Snf1 induces a delay of the metaphase to anaphase transition that is due to a defect in the correct alignment of the mitotic spindle. In particul

SUBMITTER: Tripodi F 

PROVIDER: S-EPMC5895576 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature