A standardized flow cytometry network study for the assessment of circulating endothelial cell physiological ranges.

ABSTRACT: Circulating endothelial cells (CEC) represent a restricted peripheral blood (PB) cell subpopulation with high potential diagnostic value in many endothelium-involving diseases. However, whereas the interest in CEC studies has grown, the standardization level of their detection has not. Here, we undertook the task to align CEC phenotypes and counts, by standardizing a novel flow cytometry approach, within a network of six laboratories. CEC were identified as alive/nucleated/CD45negative/CD34bright/CD146positive events and enumerated in 269 healthy PB samples. Standardization was demonstrated by the achievement of low inter-laboratory Coefficients of Variation (CV_L), calculated on the basis of Median Fluorescence Intensity values of the most stable antigens that allowed CEC identi

SUBMITTER: Lanuti P 

PROVIDER: S-EPMC5895616 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature