Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids.

ABSTRACT: The development of three-dimensional culture methods has allowed for the study of developing cortical morphology in human cells. This provides a new tool to study the neurodevelopmental consequences of disease-associated mutations. Here, we study the effects of isogenic DISC1 mutation in cerebral organoids. DISC1 has been implicated in psychiatric disease based on genetic studies, including its interruption by a balanced translocation that increases the risk of major mental illness. Isogenic wild-type and DISC1-disrupted human-induced pluripotent stem cells were used to generate cerebral organoids, which were then examined for morphology and gene expression. We show that DISC1-mutant cerebral organoids display disorganized structural morphology and impaired proliferation, which is phenocop

SUBMITTER: Srikanth P 

PROVIDER: S-EPMC5895714 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature