Quantitative characterization of all single amino acid variants of a viral capsid-based drug delivery vehicle.

ABSTRACT: Self-assembling proteins are critical to biological systems and industrial technologies, but predicting how mutations affect self-assembly remains a significant challenge. Here, we report a technique, termed SyMAPS (Systematic Mutation and Assembled Particle Selection), that can be used to characterize the assembly competency of all single amino acid variants of a self-assembling viral structural protein. SyMAPS studies on the MS2 bacteriophage coat protein revealed a high-resolution fitness landscape that challenges some conventional assumptions of protein engineering. An additional round of selection identified a previously unknown variant (CP[T71H]) that is stable at neutral pH but less tolerant to acidic conditions than the wild-type coat protein. The capsids formed by this variant cou

SUBMITTER: Hartman EC 

PROVIDER: S-EPMC5895741 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature