Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses.

ABSTRACT: The emergence and spread of Zika virus (ZIKV) presented a challenge to the diagnosis of ZIKV infections in areas with transmission of dengue (DENV) and chikungunya (CHIKV) viruses. To facilitate detection of ZIKV infections, and differentiate these infections from DENV and CHIKV, we developed the Trioplex real-time RT-PCR assay (Trioplex assay). Here, we describe the optimization of multiplex and singleplex formats of the assay for a variety of chemistries and instruments to facilitate global standardization and implementation. We evaluated the analytical performance of all Trioplex modalities for detection of these three pathogens in serum and whole blood, and for ZIKV in urine. The limit of detection for the three viruses and in different RNA-extraction modalities is near 10³

SUBMITTER: Santiago GA 

PROVIDER: S-EPMC5895813 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature