Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater.

ABSTRACT: Nucleic acid amplification of biomarkers is increasingly used to monitor microbial activity and assess remedial performance in contaminated aquifers. Previous studies described the use of filtration, elution, and direct isothermal amplification (i.e. no DNA extraction and purification) as a field-able means to quantify Dehalococcoides spp. in groundwater. This study expands previous work with direct loop mediated isothermal amplification (LAMP) for the detection and quantification of Dehalobacter spp. in groundwater. Experiments tested amplification of DNA with and without crude lysis and varying concentrations of humic acid. Three separate field-able methods of biomass concentration with eight aquifer samples were also tested, comparing direct LAMP with traditional DNA extraction and quan

SUBMITTER: Stedtfeld RD 

PROVIDER: S-EPMC5896009 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature