A leukocyte activation test identifies food items which induce release of DNA by innate immune peripheral blood leucocytes.

ABSTRACT:

Background

Leukocyte activation (LA) testing identifies food items that induce a patient specific cellular response in the immune system, and has recently been shown in a randomized double blinded prospective study to reduce symptoms in patients with irritable bowel syndrome (IBS). We hypothesized that test reactivity to particular food items, and the systemic immune response initiated by these food items, is due to the release of cellular DNA from blood immune cells.

Methods

We tested this by quantifying total DNA concentration in the cellular supernatant of immune cells exposed to positive and negative foods from 20 healthy volunteers. To establish if the DNA release by positive samples is a specific phenomenon, we quantified myeloperoxidase (MPO) in cellular supernatants.

SUBMITTER: Garcia-Martinez I 

PROVIDER: S-EPMC5896029 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature