Operational utility of the reverse-transcription recombinase polymerase amplification for detection of dengue virus.

ABSTRACT:

Background

A method for rapid detection of dengue virus using the reverse-transcription recombinase polymerase amplification (RT-RPA) was recently developed, evaluated and made ready for deployment. However, reliance solely on the evaluation performed by experienced researchers in a well-structured and well-equipped reference laboratory may overlook the potential intrinsic problems that may arise during deployment of the assay into new application sites, especially for users unfamiliar with the test. Appropriate assessment of this newly developed assay by users who are unfamiliar with the assay is, therefore, vital.

Methods

An operational utility test to elucidate the efficiency and effectiveness of the dengue RT-RPA assay was conducted among a group of researchers new to th

SUBMITTER: Tan KK 

PROVIDER: S-EPMC5896040 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature