Epithelial to mesenchymal transition induces stem cell like phenotype in renal cell carcinoma cells.

ABSTRACT:

Background

Metastatic dissemination of solid tumors is often initiated by reactivation of an embryonic development program, epithelial-to-mesenchymal-transition (EMT). EMT has been associated with acquiring invasiveness and resistance to conventional therapies. However, the precise role of EMT during renal cell carcinoma is still debatable and is under investigation. In this context, our study is designed to evaluate the role of cyclosporine (CsA) and transforming growth factor-β (TGFβ) administration in inducing EMT-like state in renal carcinoma cells. We also studied the associated phenotypic changes which may lead to tumor metastasis.

Methods

The morphological changes in renal cell carcinoma cells (A498) treated with TGF-β/CsA were observed by microscopy. Atomic force mic

SUBMITTER: Singla M 

PROVIDER: S-EPMC5896088 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature