Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China.

ABSTRACT:

Background

Haemophilus parasuis is a common porcine respiratory pathogen that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China.

Methods

We screened 143 H. parasuis isolates for antimicrobial susceptibility against six fluoroquinolone antibiotics testing by the broth microdilution method, and the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-fiel

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC5896491 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature