Estimating intraspecific genetic diversity from community DNA metabarcoding data.

ABSTRACT:

Background

DNA metabarcoding is used to generate species composition data for entire communities. However, sequencing errors in high-throughput sequencing instruments are fairly common, usually requiring reads to be clustered into operational taxonomic units (OTUs), losing information on intraspecific diversity in the process. While Cytochrome c oxidase subunit I (COI) haplotype information is limited in resolving intraspecific diversity it is nevertheless often useful e.g. in a phylogeographic context, helping to formulate hypotheses on taxon distribution and dispersal.

Methods

This study combines sequence denoising strategies, normally applied in microbial research, with additional abundance-based filtering to extract haplotype information from freshwater macroinvertebrate

SUBMITTER: Elbrecht V 

PROVIDER: S-EPMC5896493 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature