Automated analysis of cell migration and nuclear envelope rupture in confined environments.

ABSTRACT: Recent in vitro and in vivo studies have highlighted the importance of the cell nucleus in governing migration through confined environments. Microfluidic devices that mimic the narrow interstitial spaces of tissues have emerged as important tools to study cellular dynamics during confined migration, including the consequences of nuclear deformation and nuclear envelope rupture. However, while image acquisition can be automated on motorized microscopes, the analysis of the corresponding time-lapse sequences for nuclear transit through the pores and events such as nuclear envelope rupture currently requires manual analysis. In addition to being highly time-consuming, such manual analysis is susceptible to person-to-person variability. Studies that compare large numbers of cell types and con

SUBMITTER: Elacqua JJ 

PROVIDER: S-EPMC5896979 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature