Genetic and physiological basis for antibody production by Kluyveromyces marxianus.

ABSTRACT: Kluyveromyces marxianus is a thermotolerant, crabtree-negative yeast, which preferentially directs metabolism (e.g., from the tricarboxylic acid cycle) to aerobic alcoholic fermentation. Thus K. marxianus has great potential for engineering to produce various materials under aerobic cultivation conditions. In this study, we engineered K. marxianus to produce and secrete a single-chain antibody (scFv), a product that is highly valuable but has historically proven difficult to generate at large scale. scFv production was obtained with strains carrying either plasmid-borne or genomically integrated constructs using various combinations of promoters (P _MDH1 or P _ACO1 ) and secretion signal peptides (KmINUss or Scα-MFss). As the wild-type K. marxianus secretes endogenous i

SUBMITTER: Nambu-Nishida Y 

PROVIDER: S-EPMC5897269 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature