Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species.

ABSTRACT: Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing si

SUBMITTER: Pafco B 

PROVIDER: S-EPMC5897349 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature