PiRNA-like small RNAs are responsible for the maternal-specific knockdown in the ascidian Ciona intestinalis Type A.

ABSTRACT: The mRNAs stored in eggs are crucial for embryogenesis. To address functions of maternal mRNAs, we recently reported the novel method MASK (maternal mRNA-specific knockdown), which we used to specifically knockdown maternal transcripts in the ascidian Ciona intestinalis Type A. In MASK, the cis element of a maternal gene is fused with eGFP or Kaede reporter gene, and the cassette is introduced into Ciona genome by transposon-mediated transgenesis. In eggs of the transgenic lines, the maternal expression of the gene whose cis element is used for driving the reporter gene is suppressed. The zygotic expression of the gene is not suppressed, suggesting that the MASK method can distinguish between maternal and zygotic functions of a gene. Here we investigated the cis and trans factors responsib

SUBMITTER: Satoh T 

PROVIDER: S-EPMC5897368 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature