Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish.

ABSTRACT: Neutrophils are fast-moving cells essential for host immune functions. Although they primarily rely on glycolysis for ATP, isolated primary human neutrophils depend on mitochondrial membrane potential for chemotaxis. However, it is not known whether mitochondria regulate neutrophil motility in vivo, and the underlying molecular mechanisms remain obscure. Here, we visualized mitochondria in an interconnected network that localizes to the front and rear of migrating neutrophils using a novel transgenic zebrafish line. To disrupt mitochondrial function genetically, we established a gateway system harboring the CRISPR/Cas9 elements for tissue-specific knockout. In a transgenic line, neutrophil-specific disruption of mitochondrial DNA polymerase, polg, significantly reduced the ve

SUBMITTER: Zhou W 

PROVIDER: S-EPMC5897731 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature