Development of a multipurpose scaffold for the display of peptide loops.

ABSTRACT: Protein-protein interactions (PPIs) determine a wide range of biological processes and analysis of these dynamic networks is increasingly becoming a mandatory tool for studying protein function. Using the globular ATPase domain of recombinase RadA as a scaffold, we have developed a peptide display system (RAD display), which allows for the presentation of target peptides, protein domains or full-length proteins and their rapid recombinant production in bacteria. The design of the RAD display system includes differently tagged versions of the scaffold, which allows for flexibility in the protein purification method, and chemical coupling for small molecule labeling or surface immobilization. When combined with the significant thermal stability of the RadA protein, these features create a ve

SUBMITTER: Rossmann M 

PROVIDER: S-EPMC5897841 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature