Neural stem cell encapsulation and differentiation in strain promoted crosslinked polyethylene glycol-based hydrogels.

ABSTRACT: Encapsulated cell viability within crosslinked hydrogels is a critical factor to consider in regenerative medicine/cell delivery applications. Herein, a "click" hydrogel system is presented encompassing 4-dibenzocyclooctynol functionalized polyethylene glycol, a four arm polyethylene glycol tetraazide crosslinker, tethered native protein attachment ligands (laminin), and a tethered potent neurogenic differentiation factor (interferon-γ). With this approach, hydrogel formation occurs via strain-promoted, metal-free, azide-alkyne cycloaddition in an aqueous buffer. This system demonstrated safe encapsulation of neural stem cells in biological conditions without chemical initiators/ultraviolet light, achieving high cell viability. Cell viability in click gels was nearly double that of ultravi

SUBMITTER: Li H 

PROVIDER: S-EPMC5898193 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature