Generation and use of a humanized bone-marrow-ossicle niche for hematopoietic xenotransplantation into mice.

ABSTRACT: Xenotransplantation is frequently used to study normal and malignant hematopoiesis of human cells. However, conventional mouse xenotransplantation models lack essential human-specific bone-marrow (BM)-microenvironment-derived survival, proliferation, and self-renewal signals for engraftment of normal and malignant blood cells. As a consequence, many human leukemias and other hematologic disorders do not robustly engraft in these conventional models. Here, we describe a complete workflow for the generation of humanized ossicles with an accessible BM microenvironment that faithfully recapitulates normal BM niche morphology and function. The ossicles, therefore, allow for accelerated and superior engraftment of primary patient-derived acute myeloid leukemia (AML) and other hematologic maligna

SUBMITTER: Reinisch A 

PROVIDER: S-EPMC5898606 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature