Biofilm infections between Scylla and Charybdis: interplay of host antimicrobial peptides and antibiotics.

ABSTRACT:

Purpose

The aim of this study is to improve the anti-biofilm activity of antibiotics. We hypothesized that the antimicrobial peptide (AMP) complex of the host's immune system can be used for this purpose and examined the assumption on model biofilms.

Methods

FLIP7, the AMP complex of the blowfly Calliphora vicina containing a combination of defensins, cecropins, diptericins and proline-rich peptides was isolated from the hemolymph of bacteria-challenged maggots. The complex interaction with antibiotics of various classes was studied in biofilm and planktonic cultures of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii by the checkerboard method using trimethyl tetrazol

SUBMITTER: Chernysh S 

PROVIDER: S-EPMC5898886 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature