Ontology highlight
ABSTRACT:
SUBMITTER: Lee PT
PROVIDER: S-EPMC5898912 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Lee Pei-Tseng PT Zirin Jonathan J Kanca Oguz O Lin Wen-Wen WW Schulze Karen L KL Li-Kroeger David D Tao Rong R Devereaux Colby C Hu Yanhui Y Chung Verena V Fang Ying Y He Yuchun Y Pan Hongling H Ge Ming M Zuo Zhongyuan Z Housden Benjamin E BE Mohr Stephanie E SE Yamamoto Shinya S Levis Robert W RW Spradling Allan C AC Perrimon Norbert N Bellen Hugo J HJ
eLife 20180322
We generated a library of ~1000 <i>Drosophila</i> stocks in which we inserted a construct in the intron of genes allowing expression of <i>GAL4</i> under control of endogenous promoters while arresting transcription with a polyadenylation signal 3' of the GAL4. This allows numerous applications. First, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry secon ...[more]