Interaction of DJ-1 with Lyn is essential for IgE-mediated stimulation of human mast cells.

ABSTRACT:

Background

DJ-1 is a redox-sensitive protein with multiple roles in cell homeostasis, levels of which are altered in patients with mast cell (MC)-related disorders. However, whether DJ-1 can regulate human MC function is unknown.

Objective

We sought to investigate the potential role of DJ-1 in the responses of human MCs to antigen stimulation.

Methods

DJ-1 was silenced in human CD34⁺-derived MCs and in the LAD2 MC line by using lentiviral short hairpin RNA constructs. Release of β-hexosaminidase, prostaglandin D₂, and GM-CSF and changes in reactive oxygen species levels were measured after FcεRI engagement. Enzymatic assays, sucrose density gradient centrifugation, immunoprecipitation, dot and Western blotting, and confocal imaging were performed

SUBMITTER: Kim DK 

PROVIDER: S-EPMC5899056 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature