A modified sequence capture approach allowing standard and methylation analyses of the same enriched genomic DNA sample.

ABSTRACT:

Background

Bread wheat has a large complex genome that makes whole genome resequencing costly. Therefore, genome complexity reduction techniques such as sequence capture make re-sequencing cost effective. With a high-quality draft wheat genome now available it is possible to design capture probe sets and to use them to accurately genotype and anchor SNPs to the genome. Furthermore, in addition to genetic variation, epigenetic variation provides a source of natural variation contributing to changes in gene expression and phenotype that can be profiled at the base pair level using sequence capture coupled with bisulphite treatment. Here, we present a new 12 Mbp wheat capture probe set, that allows both the profiling of genotype and methylation from the same DNA sample. Furthermore, w

SUBMITTER: Olohan L 

PROVIDER: S-EPMC5899405 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature