Interleukin-8 mediates neutrophil-endothelial interactions in pig-to-human xenogeneic models.

ABSTRACT:

Background

Human neutrophils are sequestered by pig lung xenografts within minutes during ex vivo perfusion. This phenomenon is not prevented by pig genetic modifications that remove xeno-antigens or added human regulatory molecules intended to down-regulate activation of complement and coagulation pathways. This study investigated whether recipient and donor interleukin-8 (IL-8), a chemokine known to attract and activate neutrophils during inflammation, is elaborated in the context of xenogeneic injury, and whether human or pig IL-8 promote the adhesion of human neutrophils in in vitro xenograft models.

Methods

Plasma levels of pig, human or non-human primate (NHP) IL-8 from ex vivo pig lung perfusion experiments (n = 10) and in vivo pig-to-baboon lung transplantation in ba

SUBMITTER: French BM 

PROVIDER: S-EPMC5899681 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature