Creation of artificial protein-protein interactions using α-helices as interfaces.

ABSTRACT: Designing novel protein-protein interactions (PPIs) with high affinity is a challenging task. Directed evolution, a combination of randomization of the gene for the protein of interest and selection using a display technique, is one of the most powerful tools for producing a protein binder. However, the selected proteins often bind to the target protein at an undesired surface. More problematically, some selected proteins bind to their targets even though they are unfolded. Current state-of-the-art computational design methods have successfully created novel protein binders. These computational methods have optimized the non-covalent interactions at interfaces and thus produced artificial protein complexes. However, to date there are only a limited number of successful examples of computat

SUBMITTER: Yagi S 

PROVIDER: S-EPMC5899712 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature