Fosl1 overexpression directly activates trophoblast-specific gene expression programs in embryonic stem cells.

ABSTRACT: During early development in placental mammals, proper trophoblast lineage development is essential for implantation and placentation. Defects in this lineage can cause early pregnancy failures and other pregnancy disorders. However, transcription factors controlling trophoblast development remain poorly understood. Here, we utilize Fosl1, previously implicated in trophoblast giant cell development as a member of the AP-1 complex, to trans-differentiate embryonic stem (ES) cells to trophoblast lineage-like cells. We first show that the ectopic expression of Fosl1 is sufficient to induce trophoblast-specific gene expression programs in ES cells. Surprisingly, we find that this transcriptional reprogramming occurs independently of changes in levels of ES cell core factors during the cell fate

SUBMITTER: Lee BK 

PROVIDER: S-EPMC5899959 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature