Experimental study of delivery of humidified-warm carbon dioxide during open abdominal surgery.

ABSTRACT: BACKGROUND:The aim of this study was to monitor the effect of humidified-warm carbon dioxide (HWCO2 ) delivered into the open abdomen of mice, simulating laparotomy. METHODS:Mice were anaesthetized, ventilated and subjected to an abdominal incision followed by wound retraction. In the experimental group, a diffuser device was used to deliver HWCO2 ; the control group was exposed to passive air flow. In each group of mice, surgical damage was produced on one side of the peritoneal wall. Vital signs and core temperature were monitored throughout the 1-h procedure. The peritoneum was closed and mice were allowed to recover for 24 h or 10 days. Tumour cells were delivered into half of the mice in each cohort. Tissue was then examined using scanning electron microscopy and immunohistochemistry.

SUBMITTER: Carpinteri S 

PROVIDER: S-EPMC5901019 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature