Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates.

ABSTRACT: Disrupting a gene to determine its effect on an organism's phenotype is an indispensable tool in molecular biology. Such techniques are critical for understanding how a gene product contributes to the development and cellular identity of organisms. The explosion of genomic sequencing technologies combined with recent advances in genome-editing techniques has elevated the possibilities of genetic manipulations in numerous organisms in which these experiments were previously not readily accessible or possible. Introducing the next generation of molecular biologists to these emerging techniques is key in the modern biology classroom. This comprehensive review introduces undergraduates to CRISPR/Cas9 editing and its uses in genetic studies. The goals of this review are to explain how CRISPR fu

SUBMITTER: Thurtle-Schmidt DM 

PROVIDER: S-EPMC5901406 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature