Evaluation of glyphosate resistance in Arabidopsis thaliana expressing an altered target site EPSPS.

ABSTRACT:

Background

Glyphosate-resistant goosegrass has recently evolved and is homozygous for the double mutant of EPSPS (T₁₀₂ I, P₁₀₆ S or TIPS). These same mutations combined with EPSPS overexpression, have been used to create transgenic glyphosate-resistant crops. Arabidopsis thaliana (Wt EPSPS K_i ∼ 0.5 μM) was engineered to express a variant AtEPSPS-T₁₀₂ I, P₁₀₆ A (TIPA K_i = 150 μM) to determine the resistance magnitude for a more potent variant EPSPS that might evolve in weeds.

Results

Transgenic A. thaliana plants, homozygous for one, two or four copies of AtEPSPS-TIPA, had resistance (IC₅₀ values, R/S) as measured by seed production ranging from 4.3- to 16-fold. Plants treated in reproductive stage w

SUBMITTER: Sammons RD 

PROVIDER: S-EPMC5901408 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature