Combinatorial Library Screening with Liposomes for Discovery of Membrane Active Peptides.

ABSTRACT: Membrane active peptides (MAPs) represent a class of short biomolecules that have shown great promise in facilitating intracellular delivery without disrupting cellular plasma membranes. Yet their clinical application has been stalled by numerous factors: off-target delivery, a requirement for high local concentration near cells of interest, degradation en route to the target site, and in the case of cell-penetrating peptides, eventual entrapment in endolysosomal compartments. The current method of deriving MAPs from naturally occurring proteins has restricted the discovery of new peptides that may overcome these limitations. Here, we describe a new branch of assays featuring high-throughput functional screening capable of discovering new peptides with tailored cell uptake and endosomal es

SUBMITTER: Carney RP 

PROVIDER: S-EPMC5901688 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature