A novel real-time RT-PCR assay for influenza C tested in Peruvian children.

ABSTRACT:

Background

Influenza C virus (ICV) is associated with acute respiratory illness. Yet ICV remains under recognized, with most previous studies using only culture to identify cases.

Objectives

To develop a sensitive and specific real-time RT-PCR assay for ICV that allows for rapid and accurate detection in a clinical or research setting.

Study design

Multiple ICV sequences obtained from GenBank were analyzed, including 141 hemagglutinin-esterase (HE), 106 matrix (M), and 97 nucleoprotein (NP) sequences. Primers and probes were designed based on conserved regions. Multiple primer-probe sets were tested against multiple ICV strains.

Results

The ICV M and NP genes offered the most conserved sequence regions. Primers and probes based on newer sequence data offered en

SUBMITTER: Howard LM 

PROVIDER: S-EPMC5901714 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature