Co-expression of the calcitonin receptor gene in the hypothalamic kisspeptin neurons in female rats.

ABSTRACT:

Purpose

Hypothalamic kisspeptin neurons are considered to play a critical role in regulating mammalian reproduction and integrating humoral and neuronal inputs that control gonadotropin-releasing hormone (GnRH)/gonadotropin release. The present study aimed to investigate the upstream regulator candidates for kisspeptin neurons.

Methods

Visualized kisspeptin neurons that were taken from the arcuate nucleus (ARC) of Kiss1-tdTomato rats were subjected to next-generation sequencing (NGS) analysis. In situ hybridization (ISH) for the calcitonin receptor gene (Calcr) was performed throughout the whole forebrain of ovariectomized wild-type female rats that had been implanted with a negative feedback level of estrogen, because the Calcr expression was evident in

SUBMITTER: Assadullah 

PROVIDER: S-EPMC5902471 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature