Interactions Among Host-Parasite MicroRNAs During Nosema ceranae Proliferation in Apis mellifera.

ABSTRACT: We previously identified microRNA (miRNA) from Nosema ceranae and found that knockdowns of transcripts for the parasite protein Dicer greatly reduce parasite reproduction. In order to study parasitic miRNA functions and identify candidate target genes, we fed honey bees infected with N. ceranae with small interfering RNA (siRNA) targeting the N. ceranae gene Dicer. We then deep-sequenced honey bee and N. ceranae miRNAs daily across a full 6-day proliferation cycle. We found seven honey bee and five N. ceranae miRNAs that were significantly differently expressed between the infection and siRNA-Dicer groups. N. ceranae miRNA showed potentially strong impacts on the N. ceranae transcriptome, where over 79% of the total protein coding genes were significantly correlated with one or more miRNAs

SUBMITTER: Evans JD 

PROVIDER: S-EPMC5902570 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature